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Gateway? Technology

Gateway®克隆技术的基础是 λ噬菌体的位点特异性重组反应,一种保存遗传信息的有效的生化途径(见图1)。与传统的需要DNA限制性内切酶、DNA连接酶、凝胶电泳和DNA片段纯化等多个步骤的繁琐亚克隆方法相比,该方法只需一步生化反应而且操作方便、快捷(室温60分钟),省时高效(见图2)。另外,根据目的蛋白的生产和纯化的不同要求, Invitrogen公司提供一系列含有不同启动子和融合标签、以及在几种宿主表达的目标载体。有关Gateway®克隆技术的详细信息,请访问Invitrogen公司的网址:www.invitrogen.com。

 

图1. λ噬菌体的位点特异性重组反应。(点击放大) 图2. 一步法从ORFEXPRESS™ 穿梭克隆转移ORF到目的载体

 

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The purchase of ORFEXPRESS™ Gateway® Shuttle Clones is accompanied by a limited label license under U.S. Patents 5,888,732; 6,143,557; 6,171,861; 6,270,969; 6,277,608 owned by Invitrogen, Corp. only to use the product for the internal research purposes of the purchaser. For information on purchasing a license to use the purchased product for purposes other than the internal research of the purchaser, contact the Director of Licensing at Invitrogen, Corp., 1600 Faraday Avenue, Carlsbad, CA 92008.

The trademark Gateway® is owned by Invitrogen Corporation.